Purpose To characterize in vivo the transpedicular approach (TA) as an alternative route to study interverte- bral disc (IVD) regeneration strategies in a sheep model. Methods 48 IVD of 12 sheep were used. TA was per- formed under fluoroscopy, followed by nucleotomy (2-mm shaver resector). A polyurethane scaffold was used to repair the end-plate. X-ray and MRI images were acquired pre-, intra- and post-operatively (1, 3, 6 months). Com- plications were recorded.\udResults TA was feasible in all animals; nucleus pulposus (NP) from L1 to L5 was accessible. Nucleotomy followed by end-plate repair was achieved. Loss of NP signal intensity was shown in MRI images of the nucleotomy group.\udConclusions TA is feasible in vivo, repeatable after only a short learning period and safely performed without sig- nificant morbidity. This animal model allows the study of IVD degeneration physiopathology and investigation of IVD regeneration techniques in vivo.
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